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Does the Nitric Oxide Synthase T786C Gene Polymorphism Influence Arterial Stiffness in Patients with Metabolic Syndrome?

Artery Research volume 26, pages 97–101 (2020)Cite this article

Abstract

Background

Endothelial Nitric Oxide Synthase (eNOS) is responsible for Nitric Oxide (NO) bioavailability at endothelial level. Aging (even in healthy people) is involved in arterial stiffness increases.

Materials and Methods

We investigated (in the service of Cardiology, 4th Medical Clinic) 100 patients, 55 with metabolic syndrome (MS), mean age 56.91 ± 14.39 years, 66% women. Identification of the T786C polymorphism was performed by enzymatic digestion of the fragment obtained by polymerase chain reaction (PCR) amplification. Evaluation of arterial parameters (aortic pulse wave velocity (PWV), as a measure of arterial stiffness and aortic [AixAo] and brachial [Aixb] augmentation index) was performed with the TensioMed™ Arteriograph.

Results

Regarding T786C polymorphism, the distribution was the following: 57% did not have the mutation (TT), 30% were heterozygous, 13% were homozygous (CC). Patients with MS more frequently had C allele (54.5% vs. 28.9% in those without MS) and CC state (16.4% vs. 8.9%, p-NS). Significant differences (p = 0.005) regarding PWV were found in TT patients vs. heterozygous CT vs. homozygous CC: 9.75 ± 1.75 m/s vs. 9.86 ± 1.56 m/s vs. 11.65 ± 1.87 m/s. In case of the other parameters, no significant differences were found (AixAo, p = 0.35; Aixb, p = 0.22; pulse pressure, p = 0.14), but CC patients presented higher values.

Conclusion

Arterial stiffness is influenced by eNOS gene polymorphisms, being a possible link between the increase in cardiovascular risk and presence of metabolic syndrome in these patients.

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Author notes

  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Internal Medicine Department, “Iuliu Haţieganu”, University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Angela Cozma, Teodora Alexescu, Vasile Negrean, Olga Hilda Orăşan & Adela-Viviana Sitar-Taut

  2. Clinical Center of Diabetes, Nutrition, Metabolic diseases, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Adriana Fodor

  3. Department of Medical Biochemistry, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Lucia Maria Procopciuc

  4. Department of Cardiology, Clinical Rehabilitation Hospital, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Dana Pop

  5. Department of Pneumology, “Iuliu Haţieganu” University of Medicine and Pharmacy, Cluj-Napoca, Romania

    Doina Alina Todea

Authors
  1. Angela Cozma
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  2. Adriana Fodor
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  3. Lucia Maria Procopciuc
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  4. Teodora Alexescu
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  5. Vasile Negrean
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  6. Dana Pop
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  7. Doina Alina Todea
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  8. Olga Hilda Orăşan
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  9. Adela-Viviana Sitar-Taut
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Corresponding author

Correspondence to Olga Hilda Orăşan.

Additional information

Peer review under responsibility of the Association for Research into Arterial Structure and Physiology

Data availability statement: The data that support the findings of this study are available from the corresponding author (OHO), upon reasonable request.

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This is an open access article distributed under the CC BY-NC 4.0 license (http://creativecommons.org/licenses/by-nc/4.0/).

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Cozma, A., Fodor, A., Procopciuc, L.M. et al. Does the Nitric Oxide Synthase T786C Gene Polymorphism Influence Arterial Stiffness in Patients with Metabolic Syndrome?. Artery Res 26, 97–101 (2020). https://doi.org/10.2991/artres.k.200324.001

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  • DOI: https://doi.org/10.2991/artres.k.200324.001

Keywords

Artery Research

ISSN: 1876-4401

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