P77 Targeted Lipidomics of Arterial Stiffness and Hemodynamics in Atherosclerosis

Abstract

Objectives

This study examined relationships between lipidomic profile, arterial function and hemodynamics in coronary artery disease (CAD) patients, peripheral arterial disease (PAD) patients and healthy controls.

Methods

We studied 52 patients with CAD, 32 patients with PAD, and 40 apparently healthy controls. Serum levels of 40 acylcarnitines, 76 phosphatidylcholines (PC) and 14 lysophosphatidylcholines (lysoPC) were determined with the AbsoluteIDQ™ p180 kit (BIOCRATES). Arterial applanation tonometry (Sphygmocor, Atcor Medical) was used for pulse wave analysis and carotid-femoral pulse wave velocity (cf-PWV) assessments.

Results

1) Acylcarnitine profile (CAD patients vs healthy subjects): elevated levels of C16:1, C18:1, C3-DC(C4-OH), PC aa C40:6, Met-SO/Met were observed in the CAD group compared to the healthy controls. Cf-PWV showed positive correlations with C14, C16:1, (C2 + C3)/C0, C2/C0 and the CPT-1 ratio for the CAD group. Moreover, PCA-derived factor 3 (acylcarnitines) proved to be an independent determinant of cf-PWV for these patients. 2) PC and lysoPC profiles (CAD patients vs PAD patients vs healthy subjects): decreased serum levels of several PC and lysoPC species (PC aa C28:1, PC aa C30:0, PC aa C32:2, PC ae C30:0, PC ae C34:2, lysoPC a C18:2) were observed for both patient groups in comparison to the healthy controls. Further, a considerable number of PCs and lysoPCs were inversely related to either cf-PWV, heart rate, asymmetric dimethylarginine (ADMA) or ADMA/arginine only for patients.

Conclusions

In addition to classical lipid-related cardiovascular risk markers, intermediates of lipid metabolism may serve as novel indicators for altered vascular function and hemodynamics.