P4 Mechanism of Proangiogenic Activity of Mitocorrectin on Endothelial Cells in Vitro

Abstract

Background

Investigations of different effective treatment modalities of infectious and inflammatory complications of stroke remain relevant. Normalization of vascularization, impaired due to hypoxia, is an important component of ischemic disease treating. The aim of our work was to study the mechanism of action of mitocorrectin on endothelial cells in vitro.

Methods

Active ingredient of mitocorrectin is a set of oligopeptides and amino acids isolated from cell mitochondria of the liver, brain and the pancreas (10:10:1) of pigs. As an experimental model was used endothelial cell line (PAEC), which was incubated at the standard conditions. Cytotoxic/proliferative effect on cultured cells was determined using cytofluorymetric analysis and MTT-test.

Results

Our studies have shown that mitocorrectin increased of endothelial cell by 25% and decreased apoptotic cells almost 2 times compared with the control. Cytofluorymetric analysis revealed an increase 1.8-fold in the population of proliferative cells pool under the influence of mitocorrectin. The most pronounced mitogenic and antiapoptotic effect of mitocorrectin on the endothelial cells was at concentrations of 0.1 – 1/ml. Thus, these doses may be the most therapeutically effective in restoring vascularization in post-stroke period. In addition, long-term cultivation of cells in the 2D-culture when exposed to mitocorrectin, more intensive formation of the capillary-like structures compared with controls, which may indicate vascular morphogenesis.

Conclusions

Thus, a study suggests that mitocorrectin shows a positive proangiogenic effect on endothelial cell line and this drug can be quite effective to restore vascularization, which is important in post-stroke period at ischemic complications.