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Gut microbiota and vascular biomarkers in patients without clinical cardiovascular diseases
Artery Research volume 18, pages 41–48 (2017)
Abstract
The aim of this research was to study the association between the gut microbiota composition and arterial wall properties. The study included 92 participants, men and women aged 25–76 years old without clinical manifestation of chronic diseases but with the possible presence of cardiovascular risk factors. Carbohydrate metabolism examination, duplex scanning of the carotid arteries with the measurement of the intima-media thickness (IMT), the carotid-femoral pulse wave velocity (PWV) measurement, and 16S rRNA (V3–V4 regions) sequencing of the gut microbiota were performed in all participants. Higher Serratia abundance was associated with the increased IMT and CRP levels. Blautia representation was associated with IMT. Higher Bacteroides representation was associated with higher pulse wave velocity in non-diabetic subjects. Although this study had some limitations, we have demonstrated that the composition of the gut microbiota was associated both with atherosclerotic and arterial stiffness markers.
References
Kohn JC, Lampi MC, Reinhart-King CA. Age-related vascular stiffening: causes and consequences. Front Genet 2015;6:112.
Shen S, Wong CH. Bugging inflammation: role of the gut microbiota. Clin Transl Immunol 2016;5:e72.
Karlsson FH, Fak F, Nookaew I, Tremaroli V, Fagerberg B, Petranovic D, et al. Symptomatic atherosclerosis is associated with an altered gut metagenome. Nat Commun 2012;3:1245.
Gregory JC, Buffa JA, Org E, Wang Z, Levison BS, Zhu W, et al. Transmission of atherosclerosis susceptibility with gut microbial transplantation. J Biol Chem 2015;290:5647–60.
Rossi M, Campbell KL, Johnson DW, Stanton T, Vesey DA, Coombes JS, et al. Protein-bound uremic toxins, inflammation and oxidative stress: a cross-sectional study in stage 3–4 chronic kidney disease. Arch Med Res 2014;45:309–17.
Gulhan B, Turkmen K, Aydin M, Gunay M, Cikman A, Kara M. The relationship between serum oxalic acid, central hemodynamic parameters and colonization by oxalobacter formigenes in hemodialysis patients. Cardiorenal Med 2015;5:164–74.
Egshatyan L, Kashtanova D, Popenko A, Tkacheva O, Tyakht A, Alexeev D, et al. Gut microbiota and diet in patients with different glucose tolerance. Endocr Connect 2016;5:1–9.
Stein JH, Korcarz CE, Hurst RT, Lonn E, Kendall CB, Mohler ER, et al. Use of carotid ultrasound to identify subclinical vascular disease and evaluate cardiovascular disease risk: a consensus statement from the American Society of Echocardiography Carotid Intima-Media Thickness Task Force. Endorsed by the Society for Vascular Medicine. J Am Soc Echocardiogr 2008;21: 93–111. quiz 89–90.
Wilkinson IB, McEniery CM, Schillaci G, Boutouyrie P, Segers P, Donald A, et al. ARTERY Society guidelines for validation of non-invasive haemodynamic measurement devices: part 1, arterial pulse wave velocity. Artery Res 2010;4:34–40.
Caporaso JG, Kuczynski J, Stombaugh J, Bittinger K, Bushman FD, Costello EK, et al. QIIME allows analysis of high-throughput community sequencing data. Nat Methods 2010;7: 335–6.
Zhang H, Lu N, Feng C, Thurston SW, Xia Y, Zhu L, et al. On fitting generalized linear mixed-effects models for binary responses using different statistical packages. Stat Med 2011;30: 2562–72.
Lozupone C, Hamady M, Knight R. UniFrac-an online tool for comparing microbial community diversity in a phylogenetic context. BMC Bioinforma 2006;7:371.
Human Microbiome Project C. Structure, function and diversity of the healthy human microbiome. Nature 2012;486:207–14.
Turnbaugh PJ, Hamady M, Yatsunenko T, Cantarel BL, Duncan A, Ley RE, et al. A core gut microbiome in obese and lean twins. Nature 2009;457:480–4.
Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, et al. Diversity of the human intestinal microbial flora. Science 2005;308:1635–8.
Liu C, Finegold SM, Song Y, Lawson PA. Reclassification of Clostridium coccoides, Ruminococcus hansenii, Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus pro-ductus and Ruminococcus schinkii as Blautia coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov., Blautia producta comb. nov., Blautia schinkii comb. nov. and description of Blautia wexlerae sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 2008;58:1896–902.
Tuovinen E, Keto J, Nikkila J, Matto J, Lahteenmaki K. Cytokine response of human mononuclear cells induced by intestinal Clostridium species. Anaerobe 2013;19:70–6.
Liu AH, Redmon Jr AH. Endotoxin: friend or foe? Allergy Asthma Proc 2001;22:337–40.
Ou J, Carbonero F, Zoetendal EG, DeLany JP, Wang M, Newton K, et al. Diet, microbiota, and microbial metabolites in colon cancer risk in rural Africans and African Americans. Am J Clin Nutr 2013;98:111–20.
Wu GD, Chen J, Hoffmann C, Bittinger K, Chen YY, Keilbaugh SA, et al. Linking long-term dietary patterns with gut microbial enterotypes. Science 2011;334:105–8.
Cohen LJ, Kang HS, Chu J, Huang YH, Gordon EA, Reddy BV, et al. Functional metagenomic discovery of bacterial effectors in the human microbiome and isolation of commendamide, a GPCR G2A/132 agonist. Proc Natl Acad Sci U S A 2015;112: E4825–34.
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Kashtanova, D., Tkacheva, O., Popenko, A. et al. Gut microbiota and vascular biomarkers in patients without clinical cardiovascular diseases. Artery Res 18, 41–48 (2017). https://doi.org/10.1016/j.artres.2017.02.007
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DOI: https://doi.org/10.1016/j.artres.2017.02.007