Skip to main content
  • Poster Abstract Presentations
  • Open access
  • Published:

P8.11 Adipokine Dysregulation is Associated with Arterial Stiffness in a Model of Diet-Induced Obesity in Mice

Abstract

The aim of this work was to analyze vascular remodeling and mechanical alterations in mesenteric arteries in a model of diet-induced obesity in mice, as well as the impact of adipokine dysregulation in those changes. Four-week old CB57BL/6J male mice were assigned either to a control (10% kcal from fat) or to a high-fat (HF) diet (45% kcal from fat). After 32 weeks of diet, HF animals weighed 30% more than controls (p<0.001). Moreover, HF animals exhibited an increase in leptin but a reduction in adiponectin plasma levels. Studies of arterial structure and mechanics, performed by pressure myography did not reveal a significant vascular remodeling in HF mice. However, we observed a significant increase in arterial stiffness in HF mice, as assessed by b-values (obtained from stress-strain relationship; LF=2.4±0.5 vs HF=5.3±0.8, p<0.05) and pulse wave velocity values (LF=3.4±0.1 vs HF=3.9±0.1; p<0.05). Moreover, though we did not find differences in elastin content, a significant reduction in fenestrae number with HF diet together with a significant increase in collagen I amount (p<0.05) were observed. Positive and negative correlations were found between b-values and leptin or adiponectin levels, respectively (p<0.01). In conclusion, these data demonstrate that HF diet accounts for an increase in arterial stiffness that is associated with adipokine dysregulation. Supported by grants from Ministerio de Ciencia e Investigación (BFU2011-25303), Ministerio de Economía y Competitividad (SAF2009-09714, SAF2011-25303, BFU2012-35353), Grupos Universidad Complutense de Madrid (UCM;GR-921641), Fundación Universitaria San Pablo-CEU, Fundación Mutua Madrileña and Sociedad para el Estudio de la Salud Cardiometabólica (SESCAMET).

Author information

Authors and Affiliations

Authors

Rights and permissions

This is an open access article distributed under the CC BY-NC license http://creativecommons.org/licenses/by/4.0/.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gil-Ortega, M., Martín-Ramos, M., Arribas, S. et al. P8.11 Adipokine Dysregulation is Associated with Arterial Stiffness in a Model of Diet-Induced Obesity in Mice. Artery Res 8, 153–154 (2014). https://doi.org/10.1016/j.artres.2014.09.186

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1016/j.artres.2014.09.186